Relative promoter strength
Posted 26 September 2011 - 11:29 PM
Any other explanations people have for this would also be greatly appreciated.
Posted 27 September 2011 - 12:59 AM
In my experience, the CMV promoter is stronger than the EF1a promoter in 293T cells, but I never quantified this, so I'm only making an 'educated' guess. Is it the same cDNA which is cloned in the vectors you are using?
Posted 07 July 2012 - 12:57 AM
As you may know a list of factors determines the final out put, which is the amout of protein in the cell. Promoter sequence is only one of these factors.
Thus, it is highly recomemded to optimized the conditions for every single gene of interest. We know that most of the it's not possible in the lab, because of time, money and experience limitations.
But some companies do perform a screening of conditions. So it's recomended to look if any of the companies produce you protein. Some relevant details and even protocols sometimes are available.