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PCR colonies of Pichia pastoris


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#1 paramyosin

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Posted 26 September 2011 - 01:37 PM

Hello all!
Any of you know a protocol to perform a rapid colony PCR of Pichia pastoris?, I think is necessary to use liticase, because at least in my hands it is not possible to perform a colony PCR as in the case of E. coli clones, with a previous step heating the colonies.
Thank you in advance!!
"Research without indebtedness is suspect, and somebody must always, somehow, be thanked." Umberto Eco

#2 KatB

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Posted 31 January 2012 - 12:31 AM

This method works very well: http://www.ncbi.nlm....les/PMC3182553/




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