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Differences between pYC2/CT and pYC2/NT

Cloning expression s. cereviseae

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#1 astrowoon

astrowoon

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Posted 26 September 2011 - 12:37 AM

Hi, anybody knows the differences between pYC2/CT and pYC2/NT yeast cloning system? From what I know one is N terminal tagged and the other is carboxyl terminal tagged. What are the advantages/disadvantages using each of them? Please share if you have any experiences working with S. cereviseae for protein expression. Thanks!




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