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IHC Storing samples

IHC Fixing

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#1 Taminem2012

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Posted 25 September 2011 - 09:17 AM

Hi,
I have some questions regarding storing of samples midway through the protocol. Is it okay to store samples after fixing so that I can stain the next day? If so, should I store it dry or in solution? Would storing it dry affect the quality of the staining? Thanks.

#2 bob1

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Posted 25 September 2011 - 08:13 PM

It should be OK to store after fixing - after all fixing is about stopping things happening in the tissue. If you are planning on processing the samples in a short time frame then store them wet in PBS or TBS.

The storage will depend on how they are fixed and what sort of sample it is - cells on a coverslip can be stored in the -20 for a while, but blocks of mounted tissue are best stored in the fridge or at room temperature.

#3 Taminem2012

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Posted 25 September 2011 - 08:41 PM

Thanks for your reply, Bob1. I really appreciate it! :).




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