Hi,
I have some questions regarding storing of samples midway through the protocol. Is it okay to store samples after fixing so that I can stain the next day? If so, should I store it dry or in solution? Would storing it dry affect the quality of the staining? Thanks.
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#2
bob1
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Posted 25 September 2011 - 08:13 PM
It should be OK to store after fixing - after all fixing is about stopping things happening in the tissue. If you are planning on processing the samples in a short time frame then store them wet in PBS or TBS.
The storage will depend on how they are fixed and what sort of sample it is - cells on a coverslip can be stored in the -20 for a while, but blocks of mounted tissue are best stored in the fridge or at room temperature.
The storage will depend on how they are fixed and what sort of sample it is - cells on a coverslip can be stored in the -20 for a while, but blocks of mounted tissue are best stored in the fridge or at room temperature.
#3
Taminem2012
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Posted 25 September 2011 - 08:41 PM
Thanks for your reply, Bob1. I really appreciate it! 
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