When I inoculate a mutant library in MTP, and I found some parts wells grow much lower compared to others. Some wells should incubate 24 hours to reach the normal OD, but the others wells just incubate 12 hours. I just wonder if all incubate 24 hours, the protein expression levels are similar with both types of mutants.
Any suggestion is welcome!
e.coli growth curve in microtiter plate
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