I am trying to extract RNA from the drosophila larvae and pupae for microarray. I want to run it on a gel to check its purity. But when i do that there is just a single band visible on the gel. Does any one have experience with Drosophila RNA work??
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mdfenko
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an elder
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Excellent
Excellent
Posted 27 September 2011 - 08:09 AM
one of the responses in this seqanswers thread says:
"In case of drosophila, 2 close peaks at the 18 S and one smaller 28 S peak..I hope I'm right about larger the S unit size"
maybe your loading wasn't sufficient to see the 28S
"In case of drosophila, 2 close peaks at the 18 S and one smaller 28 S peak..I hope I'm right about larger the S unit size"
maybe your loading wasn't sufficient to see the 28S
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