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RNA extraction and purity from Drosophila

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#1 shldbcrzy



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Posted 23 September 2011 - 08:40 AM

I am trying to extract RNA from the drosophila larvae and pupae for microarray. I want to run it on a gel to check its purity. But when i do that there is just a single band visible on the gel. Does any one have experience with Drosophila RNA work??

#2 mdfenko


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Posted 27 September 2011 - 08:09 AM

one of the responses in this seqanswers thread says:

"In case of drosophila, 2 close peaks at the 18 S and one smaller 28 S peak..I hope I'm right about larger the S unit size"

maybe your loading wasn't sufficient to see the 28S
talent does what it can
genius does what it must
i do what i get paid to do

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