Hi,
My DNA extraction are using NaOH and a kitt of DNAzol. However when I measure the concentration of the DNA using spec at 260/280 i've got 3.57 for NaOH and 2.89 for DNAzol. My friend said it may be due to protein contamination.
So my question is: how to remove all the protein and obtain pure DNA concentration?
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Protein contamination - How to remove all protein from my DNA?
Started by syazmin, Sep 22 2011 06:03 AM
protein contamination how to DNA
3 replies to this topic
#2
96well
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Posted 22 September 2011 - 08:11 AM
Perhaps a proteinase K treatment before DNA extraction could be helpful.
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#3
phage434
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Posted 22 September 2011 - 09:26 AM
A phenol choroform extraction removes all proteins. Make sure to do a chloroform only extract to remove traces of phenol from the final product.
#4
syazmin
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Posted 22 September 2011 - 05:19 PM
Thanks for the suggestions. However, actually i don't want to use phenol-chloroform since i'm doing a research of comparing 3 different methods of DNA extraction. Besides NaOH and DNAzol, i'm also using method extraction of phenol-chloroform & isoamylic acohol. So i don't want to mix these method cause i'm afraid it will affect my results of comparison.
So is there any other suggestion of how to remove the contaminant which i assume proteins?
So is there any other suggestion of how to remove the contaminant which i assume proteins?
