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Protein contamination - How to remove all protein from my DNA?

protein contamination how to DNA

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3 replies to this topic

#1 syazmin

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Posted 22 September 2011 - 06:03 AM

Hi,
My DNA extraction are using NaOH and a kitt of DNAzol. However when I measure the concentration of the DNA using spec at 260/280 i've got 3.57 for NaOH and 2.89 for DNAzol. My friend said it may be due to protein contamination.
So my question is: how to remove all the protein and obtain pure DNA concentration?

#2 96well

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Posted 22 September 2011 - 08:11 AM

Perhaps a proteinase K treatment before DNA extraction could be helpful.
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#3 phage434

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Posted 22 September 2011 - 09:26 AM

A phenol choroform extraction removes all proteins. Make sure to do a chloroform only extract to remove traces of phenol from the final product.

#4 syazmin

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Posted 22 September 2011 - 05:19 PM

Thanks for the suggestions. However, actually i don't want to use phenol-chloroform since i'm doing a research of comparing 3 different methods of DNA extraction. Besides NaOH and DNAzol, i'm also using method extraction of phenol-chloroform & isoamylic acohol. So i don't want to mix these method cause i'm afraid it will affect my results of comparison.
So is there any other suggestion of how to remove the contaminant which i assume proteins?





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