Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Cross species hybridization-How do I deal with down regulated genes caused by mi

cross species mismatch

  • Please log in to reply
No replies to this topic

#1 Amber Olson

Amber Olson

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 21 September 2011 - 09:29 AM

HI

I have completed my cross-species microarray experiments on a 70-mer oligo slide for Giardia lamblia. I ran the GS and WB isolates on a WB isolate microarray. The two isolates have been recently sequenced. I know a lot of my down-regulated genes in the GS isolate are the result of mismatches in the WB probe for the GS mRNA. I was wondering how I would determine what down-regulated genes are reliable and which ones are due to mismatches? I have the sequences of both isolates to compare. If you know of any papers that have done similar cross species experiments with two sequenced species that would be extra helpful.

Second, my supervisor thinks I should be using a equation to estimate the hybridization temperature of the mismatched cDNA to the probe. I was wondering if anyone knew of some free software that can do these calculations.

Thanks,
Amber




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.