Cell culture problem
Posted 01 February 2003 - 07:22 PM
Clearly I can not use these cells for transfection. It really bothers me. Any suggestion or information on this will be greatly appreciated!
Posted 13 February 2003 - 11:33 AM
Posted 25 December 2004 - 11:56 PM
Posted 08 January 2005 - 11:32 AM
It sounds like these little particles could be cell derbis caused by triniziation etc. Try change to fresh media after 1 day of passaging if they disappear.
But, it sound more like a yeast contamination to me (because your media went cloudy!)
I would chuck away all media, start a fresh vial of cells, ask other colleages whether they have any contamination problems in their tissue culture and fumigate the hood (just to be at the safe side)
(it is talking from experience...)
Hope it helps,