Posted 20 September 2011 - 11:39 PM
I'm new to the MTT assay and from what I've read, I have to first determine the optimal cell count before performing the assay with my test sample. None of the protocols that I've read for this [inlcuding the ATCC protocol attached], ask you to add any cytotoxic compound before adding the MTT. How then will the formazan crystals be formed if I'm going to plate cells with >90% viability? Or am I missing out on something?
Posted 21 September 2011 - 07:23 AM
Also, please do not hesitate to contact Promega Technical Services with any questions you may have.
Posted 21 September 2011 - 09:14 AM