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Mouse primary myoblast culture - appearance?

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#1 assembler01

assembler01

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Posted 20 September 2011 - 02:50 PM

Does anyone have photos of primary mouse myoblast culture at various stages? Are they supposed to be fibroblast like or do they remain as little spheres for a while (while the fibroblasts in the culture quickly adhere and spread)? Does it make a difference what you plate them on as some different protocols suggest gelatin, collagen, ECL and similar matrices? We're going to try differentiating some more as our first attempt was unsuccessful. I don't have the desmin antibody normally used to identify them by immunofluorescence, but would antibodies for Pax7, MyoD, Myf5 or myogenin work? Any others?

Thanks.





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