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fluorescent -transfection problem

fluorescent transfection

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#1 ATP

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Posted 20 September 2011 - 01:38 PM

Hi!
While transfection of cells with a plasmid containing the GFP gene, I observed cells under a fluorescent microscope, bright fluorescence as well as the damped. Can somebody help me, hence the difference?
Thanks for help,
ATP

#2 bob1

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Posted 20 September 2011 - 03:27 PM

Do you mean that you saw fluorescence in the untransfected as well as the transfected? If so, you probably have either autofluorescence or didn't wash out the fixative enough.





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