Hi!
While transfection of cells with a plasmid containing the GFP gene, I observed cells under a fluorescent microscope, bright fluorescence as well as the damped. Can somebody help me, hence the difference?
Thanks for help,
ATP
0
1 reply to this topic
#2
bob1
-
- Global Moderators
-
- 4,340 posts
Thelymitra pulchella
222
Excellent
Excellent
Posted 20 September 2011 - 03:27 PM
Do you mean that you saw fluorescence in the untransfected as well as the transfected? If so, you probably have either autofluorescence or didn't wash out the fixative enough.
