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Western Blot


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#1 luhzh

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Posted 30 January 2003 - 11:44 AM

Hi Friends,

If my tissue lysate is from mouse, I think the anti-mouse Ab as first Ab can not be used to do Western Blot,otherwise cross reactivity will happen. The better is that I need to chose anti-rabbit Ab. Is that true?
I hope to get your answer asap. Thank you so much!

Derlin

#2 skuba

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Posted 14 February 2003 - 06:53 AM

I am not a great specialist, but I think you can use anti -mouse. It depends on the specificity and crossreaktivity, yours antibodies have, you could know it.
Ira




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