How much cytoplasmic and nuclear extracts load in a WB?
Posted 20 September 2011 - 12:53 PM
I'm trying to assess protein expression in nuclear and cytoplasmic fractions. I was recommended to load three times more total protein of cytoplasmic than nuclear extracts. This was because nuclear proteins are more concentrated in the nuclear fraction. In literature I've seen that is more frecuent to load equal amounts of both extracts. In your opinion what is more appropiate?
Posted 20 September 2011 - 02:04 PM
Posted 20 September 2011 - 05:06 PM