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Can I use different cDNA concentration of my negative control for rt-pcr

rt-pcr cDNA concentration

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#1 kubic

kubic

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Posted 20 September 2011 - 09:04 AM

Hi everyone

I isolated RNA from my drug treated cell and converted it to cDNA. I'used 5ng/ul cDNA for rt-pcr. Now I 'll do rt-pcr for my negative control but I don't have that much cDNA from my control. Can I use less concentration of cDNA. Would it be problem when I'm comparing the results. Is there any calculation for this? I also like to ask that what ct values I should have to say rt-pcr worked and reliable.

Thank you very much in advance





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