has anyone tried using syto9 to stain for DNA in gels?
ie, mix some syto 9 to your PCR rxn, incubate for 5 mins, load on to gel
syto9 for DNA gel electrophoresissyto 9 DNA gel
1 reply to this topic
Posted 20 September 2011 - 03:25 PM
Does it actually bind to the DNA or is it intercalated (similar to EtBr and Sybr)? If it actually binds, it should be OK; otherwise you would need to check whether it will migrate in the same direction as the DNA on a gel.