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syto9 for DNA gel electrophoresis

syto 9 DNA gel

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#1 uginz

uginz

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Posted 19 September 2011 - 09:00 PM

Hi there
has anyone tried using syto9 to stain for DNA in gels?
ie, mix some syto 9 to your PCR rxn, incubate for 5 mins, load on to gel

#2 bob1

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Posted 20 September 2011 - 03:25 PM

Does it actually bind to the DNA or is it intercalated (similar to EtBr and Sybr)? If it actually binds, it should be OK; otherwise you would need to check whether it will migrate in the same direction as the DNA on a gel.




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