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[crimea]

Hi! I am trying to assay by western blotting NFkB, a trascription factor that translocate to the nucleus, in the brain. I need both cytoplasmic and nuclear extracts.
I have always problems of contamination of the nuclear extract with cytoplasmic fraction. What can I do? and what is the critical step?
thanks

[ramakn]

In our lab to obtain nuclear extracts and cytoplasmic extracts we use a nuclear extract kit from active motif and has not given us any contamination. Try it