Hi! I am trying to assay by western blotting NFkB, a trascription factor that translocate to the nucleus, in the brain. I need both cytoplasmic and nuclear extracts.
I have always problems of contamination of the nuclear extract with cytoplasmic fraction. What can I do? and what is the critical step?
thanks
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nuclear extract
Started by crimea, Jan 30 2003 01:49 AM
1 reply to this topic