Posted 18 September 2011 - 09:04 PM
I want to learn if trypsin (after cell detachment) could be deactivated by DMEM only without serum??
What really stops trypsin? Ions? Proteins?
Posted 19 September 2011 - 04:27 AM
apparently it is due to specific trypsin inhibitors present in serum. I am sure you can buy these inhibitors separately if you really don't want serum in your sample but seru would be by far the easiest and cheapest way.
Posted 19 September 2011 - 04:11 PM
Yes I think so that serum is the easiest way for that...
Posted 19 September 2011 - 05:09 PM