4 replies to this topic

[SelcenC]

Hi,

I want to learn if trypsin (after cell detachment) could be deactivated by DMEM only without serum??

What really stops trypsin? Ions? Proteins?

Thanks

[philman]

a little googling and I found this: http://www.ncbi.nlm....pubmed/17211556

apparently it is due to specific trypsin inhibitors present in serum. I am sure you can buy these inhibitors separately if you really don't want serum in your sample but seru would be by far the easiest and cheapest way.

[SelcenC]

Thank you for the reply

Yes I think so that serum is the easiest way for that...

[bob1]

Soy-bean trypsin inhibitor (try Sigma-Aldrich) is another way to do it, if you really have to exclude serum.

[SelcenC]

Hi Bob,

Thank you for your advice !