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Trypsin Deactivation


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4 replies to this topic

#1 SelcenC

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Posted 18 September 2011 - 09:04 PM

Hi,

I want to learn if trypsin (after cell detachment) could be deactivated by DMEM only without serum??

What really stops trypsin? Ions? Proteins?

Thanks

- Selcen -


#2 philman

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Posted 19 September 2011 - 04:27 AM

a little googling and I found this: http://www.ncbi.nlm....pubmed/17211556

apparently it is due to specific trypsin inhibitors present in serum. I am sure you can buy these inhibitors separately if you really don't want serum in your sample but seru would be by far the easiest and cheapest way.

#3 SelcenC

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Posted 19 September 2011 - 04:11 PM

Thank you for the reply :)

Yes I think so that serum is the easiest way for that...

- Selcen -


#4 bob1

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Posted 19 September 2011 - 05:09 PM

Soy-bean trypsin inhibitor (try Sigma-Aldrich) is another way to do it, if you really have to exclude serum.

#5 SelcenC

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Posted 26 September 2011 - 08:35 PM

Hi Bob,

Thank you for your advice !
:)

- Selcen -





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