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cDNA shelf-life


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1 reply to this topic

#1 Curtis

Curtis

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Excellent

Posted 18 September 2011 - 07:41 PM

Hi,

For how long can we store cDNA? I ran PCR with a cDNA that I synthesized in 2009 and it doesn't work. I have no more sample. I have to propagate more virus, extract RNA and synthesize new cDNA.

#2 allynspear

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Good

Posted 21 September 2011 - 11:59 AM

If cDNA is run through a clean up kit (any PCR or reaction cleanup kit should work) and stored in 1X TE at -20 degrees, I have used it for up to 5 years later. Alternatively, you can phenol:chloroform extract your reaction and perform an ethanol precipitation, then resuspend in TE.




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