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Staining Cells in Suspension


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#1 dgprotocol

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Posted 18 September 2011 - 02:28 PM

Hi all

Are there any protocols available for staining cells which can be done entirely in suspension (i.e. never smeared)? I would like prefer something with similar results to H&E staining.

Thank you for your time,

Daniel

#2 PAO_ahac

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Posted 19 September 2011 - 11:21 AM

trypan blue for cell viability others for flow cytometry

#3 bob1

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Posted 19 September 2011 - 05:33 PM

Hi all

Are there any protocols available for staining cells which can be done entirely in suspension (i.e. never smeared)? I would like prefer something with similar results to H&E staining.

Thank you for your time,

Daniel

How would you look at them if they don't make it onto a slide at some point?

#4 PAO_ahac

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Posted 20 September 2011 - 02:19 AM

I am only familiar with trypan blue. You use a hemocytometer...live cells you will only see outline...dead cells will stain darkly with blue color. You will have to do research to check out reagents used for flow cytometry.

#5 dgprotocol

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Posted 23 September 2011 - 11:58 AM

How would you look at them if they don't make it onto a slide at some point?


I'll take pictures of them flowing through a microchannel.

To reword my question, would it be possible to adapt an H&E staining protocol to cells which are suspended. Could I simply exchange the solutions about the cells in suspension (spin/resuspend)? Also, which steps are most critical for good Hematoxylin staining? I don't seem to be getting nuclear definition.




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