4 replies to this topic

[dgprotocol]

Hi all

Are there any protocols available for staining cells which can be done entirely in suspension (i.e. never smeared)? I would like prefer something with similar results to H&E staining.

Thank you for your time,

Daniel

[PAO_ahac]

trypan blue for cell viability  others for flow cytometry

[bob1]

dgprotocol, on 18 September 2011 - 02:28 PM, said:

Hi all

Are there any protocols available for staining cells which can be done entirely in suspension (i.e. never smeared)? I would like prefer something with similar results to H&E staining.

Thank you for your time,

Daniel

How would you look at them if they don't make it onto a slide at some point?

[PAO_ahac]

I am only familiar with trypan blue.  You use a hemocytometer...live cells you will only see outline...dead cells will stain darkly with blue color. You will have to do research to check out reagents used for flow cytometry.

[dgprotocol]

Quote

How would you look at them if they don't make it onto a slide at some point?

I'll take pictures of them flowing through a microchannel.

To reword my question, would it be possible to adapt an H&E staining protocol to cells which are suspended. Could I simply exchange the solutions about the cells in suspension (spin/resuspend)? Also, which steps are most critical for good Hematoxylin staining? I don't seem to be getting nuclear definition.