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Northern v. RPA v. RT-qPCR


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#1 labbey2.0



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Posted 16 September 2011 - 06:22 AM

Hello, everyone. I'm writing my Master's thesis proposal, and I was trying to decide to which alternative method to propose for quantifying RNA.

Our lab studies viral RNA replication, and we quantify this via Northern blot. I've read somewhere that a drawback of the Northern is that you require large quantities of RNA (very true, haha). As such, I'd like to propose an alternative method for my research.

I was wondering if anyone could direct me toward some references that discuss the pros and cons of RNAse protection assays vs. reverse-transcription quantitative PCR. Is one assay truly more sensitive to quantification than the other? Do your labs use one technique over the other?

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