Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Northern v. RPA v. RT-qPCR

Quantification

  • Please log in to reply
No replies to this topic

#1 labbey2.0

labbey2.0

    member

  • Active Members
  • Pip
  • 5 posts
0
Neutral

Posted 16 September 2011 - 06:22 AM

Hello, everyone. I'm writing my Master's thesis proposal, and I was trying to decide to which alternative method to propose for quantifying RNA.

Our lab studies viral RNA replication, and we quantify this via Northern blot. I've read somewhere that a drawback of the Northern is that you require large quantities of RNA (very true, haha). As such, I'd like to propose an alternative method for my research.

I was wondering if anyone could direct me toward some references that discuss the pros and cons of RNAse protection assays vs. reverse-transcription quantitative PCR. Is one assay truly more sensitive to quantification than the other? Do your labs use one technique over the other?





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.