Hello, everyone. I'm writing my Master's thesis proposal, and I was trying to decide to which alternative method to propose for quantifying RNA.
Our lab studies viral RNA replication, and we quantify this via Northern blot. I've read somewhere that a drawback of the Northern is that you require large quantities of RNA (very true, haha). As such, I'd like to propose an alternative method for my research.
I was wondering if anyone could direct me toward some references that discuss the pros and cons of RNAse protection assays vs. reverse-transcription quantitative PCR. Is one assay truly more sensitive to quantification than the other? Do your labs use one technique over the other?
No replies to this topic
Also tagged with one or more of these keywords: Quantification
Protocols and Techniques Forums →
SDS-PAGE and Western Blotting →
Fold-changes different when quantifying same protein via IF and WesternStarted by Guest_Caribou_* , 19 Jun 2012 |
|
|
||
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
Amplicon side productsStarted by Guest_Le_Bioguy_* , 20 Feb 2012 |
|
|
||
Protocols and Techniques Forums →
Molecular Biology →
RNA quantification (in pmol)Started by Guest_DRYTCYV_* , 13 Jan 2012 |
|
|
||
Protocols and Techniques Forums →
Molecular Biology →
Quantifying DNA from Buccal SwabsStarted by Guest_ca.dreaming_* , 13 Oct 2011 |
|
|













