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Environmental DNA extractions

Started by Chatnoir, Sep 16 2011 02:00 AM

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#1 Chatnoir

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Posted 16 September 2011 - 02:00 AM

Hi,

can anybody advise any alterations to a normal CTAB DNA extraction, that is more sensitive to environmental anammox bacteria DNA?

thanks!

#2 akhshik

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Posted 27 December 2011 - 12:51 AM

there are some sort of Nano Magnetic DNA extraction kits out there, but I think CTAB is more friendly for our environment.

#3 ickypimp

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Posted 02 February 2012 - 06:04 PM

Hi try a ctab/polyvinylpyrrolidone extraction, the PVPP is excellent at mopping up polyphenols and carbohydrates from environmental samples which can be inhibitory to enzymatic reactions

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