Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

stable transfection suspension cultures


  • Please log in to reply
1 reply to this topic

#1 ermmh

ermmh

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 15 September 2011 - 12:10 PM

Can anyone comment on their favorite method for transfection of suspension cultures and the levels of stable integration? I'm interested in CHO, but comments on other cells are welcome, too. Is there any info on the levels of integration when using Electroporation vs lipofection. Nucleofection vs other systems, etc?

I appreciate your comments. Thanks.

#2 protolder

protolder

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 293 posts
9
Neutral

Posted 15 September 2011 - 10:10 PM

Hola, check polietilenimine (Jet-Pei) for me is efficient cheap and easy to use. There are some types for each cell type, look for the most adequated for yours.http://www.biomol.de...infos_nr118.pdf




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.