Could anybody help me?
I do Western blots, and am now using nitrocellulose membranes (Schleier & Schuell). Somehow, although I'm using the same antibodies as before, the signal is most of the time very low, even with extended illumination times. We've been testing through various types of blocking reagents (dry milk, casein,...) so that's unlikely to be the cause of the problem. Could it be the type of membrane we are using. I had good experiences in the past with PVDF.
Western blot doesn't work anymore
1 reply to this topic