I've recently cloned two genes into the inducible overexpression vector 'pTRE-TIGHT'.
I've had the whole vector sequenced and it's come back with three point mutations, all residing in 'Fragment containing COL E1 origin of replication'.
I'm wondering if these mutations will have any effect on the overall function of the vector. Or if it's ok to just go on and use?
Edited by andyg2886, 13 September 2011 - 02:56 AM.