Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Protein purification buffer


  • Please log in to reply
3 replies to this topic

#1 Chelo

Chelo

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 93 posts
3
Neutral

Posted 12 September 2011 - 02:20 AM

Hello out there!

I would like to avoid Tris or Hepes in my protein purification buffer. Do you know if I could replace it by phosphate buffer? If yes, what should be the exact composition of the phosphate buffer (i.e. monobasic, mono + dibasic, concentration, pH).
Thanks a lot!

#2 K.B.

K.B.

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 174 posts
3
Neutral

Posted 12 September 2011 - 08:49 AM

I can do this for you - what pH and concentration you require? Do you need specific ionic strength? What would be the temperature you're planning to use this buffer at?

#3 newborn

newborn

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 71 posts
2
Neutral

Posted 16 September 2011 - 06:30 AM

You can purchase some PBS buffer pill from Sigma, it is very convenient.

#4 u03cka

u03cka

    member

  • Active Members
  • Pip
  • 5 posts
0
Neutral

Posted 07 December 2011 - 07:41 AM

I like MES.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.