Posted 11 September 2011 - 11:39 PM
Your advice would be much appreciated. One of the genes I am working with (imprinted gene) is expressed at extremely low levels, so reat time qRT-PCR fails to detect any transcripts. Can anyone suggest any other ways to detect few transcripts (other than performing pre-amplifaction)?? Is there any other PCR method (nested, conventional etc) that is more reliable than real time qRT-PCR?
Thank you very much
Posted 12 September 2011 - 07:12 AM
Posted 12 September 2011 - 02:36 PM