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In a pcr why doesnt the DNA reneal during annealing process ?


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#1 bochum

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Posted 10 September 2011 - 10:41 AM

During a pcr when the DNA melts and temperature is brought to 50 and the primer binds the single straned DNA,then why doesnt the original DNA reneal too when the temperate is brought to 50.
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Martin
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#2 phage434

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Posted 10 September 2011 - 10:57 AM

It does, but the molar amount of primer is vastly higher than the template DNA concentration, so sites are likely to have the primer rather than reanneal.

#3 bochum

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Posted 11 September 2011 - 06:50 AM

I thought before the original strand of DNA bind to DNA, primer bind to it because of small size of primer and more over annealing time is very short for original strand to bind to it. Once the primer to bind to DNA it is difficult for the other strand to bind to it..IS it my view is right
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Martin
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