Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

dissociation constant by ELISA


  • Please log in to reply
No replies to this topic

#1 AnetteM



  • Active Members
  • Pip
  • 14 posts

Posted 07 September 2011 - 12:56 PM

I want to determine Kd for a protein/protein interaction by ELISA. The problem is that the interaction is weak so I can not do an experiment where I let my proteins reach equillibrium in solution and thereafter meassure the unbound fraction as the proteins will dissociate directly.

I was under the assumption that I could determine Kd for a protein/protein interaction by coating one on an ELISA plate followed by titration of the other and Kd would be at the concentration corresponding to half the maximum response. Am I totaly wrong?

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.