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How to kill HRP ?

western blot

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3 replies to this topic

#1 postit

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Posted 06 September 2011 - 05:27 AM

Hi,

I Would like to kill HRP on my bot, how can i do ?
(I don't want to strip)
Thanks in advance,

#2 Lindsay Reese

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Posted 06 September 2011 - 12:21 PM

Try including 0.05% sodium azide in the solution for your next primary. If the signal wasn't blaring on the first go-round, this should do the trick.

#3 bochum

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Posted 07 September 2011 - 11:10 AM

Good idea, Do you know how it works
With Regards
Martin
Career In Life Science

#4 Gangwolf

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Posted 04 January 2012 - 07:33 AM

Or try incubating your blot in 0.4M NaOH for 20 min in room temp. on a shaker.





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