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Storage buffer for antibodies

Antibodies

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3 replies to this topic

#1 nainiburman21

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Posted 05 September 2011 - 10:32 PM

Hi,
I am planning to do chromatin immunoprecipation and had given order for antibodies. I want to know answers to the following questions


Do you want 0.05% Sodium Azide to be added in the antisera Yes NO
Composition of formulation buffer for antibody: PBS-Glycerol PBS-Glycerol-Azide PBS-Azide PBS Others
Do you want to replace PBS with Tris? YES NO

What are these buffers and when shpuld they be used for storing antibodies?

Thanks
Naini

#2 linpaco

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Posted 05 September 2011 - 11:56 PM

PBS-glycerol-Azide will be best to store antibody. keep Azide as low as you can maybe 0.01% is also sufficient. No need to add tris a PBS is a buffer of its own.

1X PBS (Phosphate Buffer Saline) + 20% Glycerol + 0.01% Sodium Azide

Edited by linpaco, 06 September 2011 - 12:03 AM.


#3 BioMiha

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Posted 06 September 2011 - 02:34 AM

I agree with linpaco PBS glycerol Azide is best for your application.

#4 Epinutrition

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Posted 06 September 2011 - 04:53 AM

i agree with two guys.





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