3 replies to this topic

[nainiburman21]

Hi,
I am planning to do chromatin immunoprecipation and had given order for antibodies. I want to know answers to the following questions


Do you want 0.05% Sodium Azide to be added in the antisera   Yes       NO
Composition of formulation buffer for antibody:  PBS-Glycerol    PBS-Glycerol-Azide       PBS-Azide  PBS      Others
Do you want to replace PBS with Tris? YES      NO

What are these buffers and when shpuld they be used for storing antibodies?

Thanks
Naini

[linpaco]

PBS-glycerol-Azide will be best to store antibody. keep Azide as low as you can maybe 0.01% is also sufficient. No need to add tris a PBS is a buffer of its own.

         1X PBS (Phosphate Buffer Saline) + 20% Glycerol + 0.01% Sodium Azide

Edited by linpaco, 06 September 2011 - 12:03 AM.

[BioMiha]

I agree with linpaco PBS glycerol Azide is best for your application.

[Epinutrition]

i agree with two guys.