I´m trying to clone some genes in a plant vector, at the time that I use the CIAP enzyme in order not to obtain religation and the T4polimerase to fill up the genes with dNTPs so I can put the genes into the vector. When I transform my ligation reaction using some E.coli colonies, at the next day I don´t have any colony grow. What I´m doing wrong?
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Cloning genes into plant vector
Started by alecandy28, Sep 02 2011 07:43 AM
plant cloning genes vector
1 reply to this topic
Also tagged with one or more of these keywords: plant, cloning, genes, vector
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