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Cloning genes into plant vector

plant cloning genes vector

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#1 alecandy28

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Posted 02 September 2011 - 07:43 AM

I´m trying to clone some genes in a plant vector, at the time that I use the CIAP enzyme in order not to obtain religation and the T4polimerase to fill up the genes with dNTPs so I can put the genes into the vector. When I transform my ligation reaction using some E.coli colonies, at the next day I don´t have any colony grow. What I´m doing wrong?

#2 phage434

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Posted 02 September 2011 - 04:00 PM

Absolutely impossible to help without MUCH more information.





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