I am new here and I need your help. I am thinking to do a project concerning antigen presentation of dendritic cell. But I do not know how I should load DC with peptide. Is it enough to add peptide in medium? should ı use serum or not? What kind of things should I consider esp .peptide design and medium ingredients? Please help me. If you have any protocol for loading DC with antigen and share here, İt will be so good ...
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