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loading dendritic cell with antigen

antigen presentatio dendritic cell

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#1 damla5n1k

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Posted 01 September 2011 - 10:13 AM

Hi everybody,
I am new here and I need your help. I am thinking to do a project concerning antigen presentation of dendritic cell. But I do not know how I should load DC with peptide. Is it enough to add peptide in medium? should ı use serum or not? What kind of things should I consider esp .peptide design and medium ingredients? Please help me. If you have any protocol for loading DC with antigen and share here, İt will be so good ...

#2 mike.sh

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Posted 04 September 2011 - 11:49 PM

Usually it is enough to add peptide to medium. But one should optimize loading time and %serum (you can start with overnight/ 5%FBS). First you can try loading with GFP and check it by flow cytometry.




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