Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Cell Lysis and Western Blotting

Cell lysis THP1

  • Please log in to reply
1 reply to this topic

#1 Eimear



  • Members
  • Pip
  • 1 posts

Posted 01 September 2011 - 04:16 AM


I need to lyse THP1 monocytes and then use western blotting to quantify the levels of certain proteins in the lysate. I tried lysing the cells with RIPA buffer, but the high detergent concentration was incompatable with running a gel. I then tried Lamberts Break buffer, but the total protein concentration of my lysare was extremely low (i.e. the cells didnt lyse).

Does anyone have any suggestions ass to how I can successfully lyse THP1s in a buffer that is compatable with SDS PAGE??

Thank you in advance!!




  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 217 posts

Posted 01 September 2011 - 06:26 AM

I dont think RIPA would interfere your gel run!! post your RIPA composition and also tell how it interferes in the running of lysate...
I would prefer being perfectionist rather than a passionist in Research.

I always had an alternate hypothesis....

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.