30 August 2011 - 12:39 PM
i am phd student
i am working on p19 cell lines
in the beginning of my first year the p19 cell line was supper confluent nearly 80-90% within 24hr and they were in a good condition
however, nowadays i have problem growing them. they start growing very slowly and they detached from the flask easily.
i have not change the medium alphaMEM with boven calf serum 7.5% and FBS 2.5%
i do not know why they start behave this way
any recommendation or opinion about what may go wrong with p19 cell line
thank you in advance
problem with embryo carcinoma cell line , p19 ,
Posted 01 September 2011 - 03:04 AM
Posted 01 September 2011 - 11:32 PM
I always had an alternate hypothesis....