
protein precipitation
#1
Posted 16 January 2003 - 06:56 PM
Many thanks,
Alberto
#2
Posted 05 March 2003 - 07:59 AM
I had the same problem. I tried with a second acetone precipitation and the salt sediment was almost away and the electrophoresis worked very good.
I hope that can help you.
Viviana
#3
Posted 12 June 2003 - 05:57 AM
I hope that can help you
hi
as usual we have two or three acetone precipitation ,if salt of solution is high,it is a good way to use TCA precipitation,
try it
#4
Posted 12 June 2003 - 05:58 AM
hihi
as usual we have two or three acetone precipitation ,if salt of solution is high,it is a good way to use TCA precipitation,
try it
as usual we have two or three acetone precipitation ,if salt of solution is high,it is a good way to use TCA precipitation,
try it
#5
Posted 18 September 2004 - 07:10 PM
if so, loss much protein,don't you?as usual we have two or three acetone precipitation ,if salt of solution is high,it is a good way to use TCA precipitation,
try it
#6
Posted 21 September 2004 - 07:07 PM
Did you use the gel for western blot? I read a paper using 300ug/lane, I really don't know how to get such high concentration of protein. Hope the percipitation will help!Hi Alberto,
I had the same problem. I tried with a second acetone precipitation and the salt sediment was almost away and the electrophoresis worked very good.
I hope that can help you.
Viviana