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protein precipitation


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5 replies to this topic

#1 aciccia76

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Posted 16 January 2003 - 06:56 PM

I precipitates my protein with acetone, but I'd like to get rid of salts as the gel is not migrating well. What can I use to wash the pellet?

Many thanks,
Alberto

#2 vaires

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Posted 05 March 2003 - 07:59 AM

Hi Alberto,

I had the same problem. I tried with a second acetone precipitation and the salt sediment was almost away and the electrophoresis worked very good.
I hope that can help you.

Viviana

#3 withmi

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Posted 12 June 2003 - 05:57 AM

I had the same problem. I tried with a second acetone precipitation and the salt sediment was almost away and the electrophoresis worked very good.
I hope that can help you
hi
as usual we have two or three acetone precipitation ,if salt of solution is high,it is a good way to use TCA precipitation,
try it

#4 withmi

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Posted 12 June 2003 - 05:58 AM

hi
as usual we have two or three acetone precipitation ,if  salt of solution is high,it is a good way to use TCA precipitation,
try it

hi
as usual we have two or three acetone precipitation ,if salt of solution is high,it is a good way to use TCA precipitation,
try it

#5 zhaoliang

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Posted 18 September 2004 - 07:10 PM

as usual we have two or three acetone precipitation ,if  salt of solution is high,it is a good way to use TCA precipitation,
try it

if so, loss much protein,don't you?

#6 lilian_gz

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Posted 21 September 2004 - 07:07 PM

Hi Alberto,

I had the same problem. I tried with a second acetone precipitation and the salt sediment was almost away and the electrophoresis worked very good.
I hope that can help you.

Viviana

Did you use the gel for western blot? I read a paper using 300ug/lane, I really don't know how to get such high concentration of protein. Hope the percipitation will help!




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