5 replies to this topic

[aciccia76]

I precipitates my protein with acetone, but I'd like to get rid of salts as the gel is not migrating well. What can I use to wash the pellet?

Many thanks,
Alberto

[vaires]

Hi Alberto,

I had the same problem. I tried with a second acetone precipitation and the salt sediment was almost away and the electrophoresis worked very good.
I hope that can help you.

Viviana

[withmi]

I had the same problem. I tried with a second acetone precipitation and the salt sediment was almost away and the electrophoresis worked very good.
I hope that can help you
hi
as usual we have two or three acetone precipitation ,if  salt of solution is high,it is a good way to use TCA precipitation,
try it

[withmi]

withmi, on Jun 12 2003, 05:57 AM, said:

hi
as usual we have two or three acetone precipitation ,if  salt of solution is high,it is a good way to use TCA precipitation,
try it

hi
as usual we have two or three acetone precipitation ,if  salt of solution is high,it is a good way to use TCA precipitation,
try it

[zhaoliang]

withmi, on Jun 12 2003, 06:57 AM, said:

as usual we have two or three acetone precipitation ,if  salt of solution is high,it is a good way to use TCA precipitation,
try it

if so, loss much protein,don't you?

[lilian_gz]

vaires, on Mar 5 2003, 08:59 AM, said:

Hi Alberto,

I had the same problem. I tried with a second acetone precipitation and the salt sediment was almost away and the electrophoresis worked very good.
I hope that can help you.

Viviana

Did you use the gel for western blot?  I read a paper using 300ug/lane, I really don't know how to get such high concentration of protein.  Hope the percipitation will help!