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Isolating plasma from frozen blood samples

Plasma whole blood

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#1 bdorresteijn

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Posted 30 August 2011 - 06:17 AM

Hello

Question. I have frozen blood samples of mice. I need to isolate the blood plasma for protein detection. Does anyone know of a protocol to do this. First I thawed the whole blood sample, which is in EDTA coated vials. I tried spinning down at 3000 rpm for 10' but no clear plasma fraction was present. Also after spinning 14000rpm for 5' no clear fractionation.
Do I need to add something, or did the freezing to -80 already mess up my experiment.

hope you can help

#2 PAO_ahac

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Posted 13 September 2011 - 02:27 AM

You lysed the blood cells by freezing. You should have spun the samples after collection to obtain the plasma before freezing. You can check your 'detection' method with a couple of samples of known concentrations and run them lysed and non-lysed to determine if your stored samples are of any value.





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