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Western Blot Protein Length

western blot protein length

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4 replies to this topic

#1 rayman

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Posted 30 August 2011 - 04:33 AM

Hello Forum,

please take a look at this image

http://imageshack.us...s/98/0bigg.jpg/

It shows a Western blot of an extract of proteins.

My question is how can you determine the length of both proteins A and B graphically?

Could you please help me?

#2 bob1

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Posted 30 August 2011 - 04:08 PM

Hmmm... homework?

What are size standards for?

#3 Papaver

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Posted 30 August 2011 - 10:40 PM

1) determine the migration distance of each standard. It starts at "Auftragspunkte der Probe"; e.g.: 180 kDa = 0.9 cm; 120 kDa = 2 cm ...
2) plot values on a semi-logarithmic scale (y-axis: logarithmic=kDa; x-axis: linear= migration distance)
3) determine the migration distance of the protein bands
4) now you can determine the size of the proteins by reference of the graph of point 2)

#4 rayman

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Posted 31 August 2011 - 04:22 AM

Thank you.

But the x-axis has to be the logarithm of the molecular weight and the y-axis has to be the migrations distance.

Edited by rayman, 31 August 2011 - 04:22 AM.


#5 bob1

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Posted 31 August 2011 - 05:41 PM

Oh noes... it's not that hard to switch the axes...





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