4 replies to this topic

[rayman]

Hello Forum,

please take a look at this image

http://imageshack.us...s/98/0bigg.jpg/

It shows a Western blot of an extract of proteins.

My question is how can you determine the length of both proteins A and B graphically?

Could you please help me?

[bob1]

Hmmm... homework?

What are size standards for?

[Papaver]

1) determine the migration distance of each standard. It starts at "Auftragspunkte der Probe"; e.g.: 180 kDa = 0.9 cm; 120 kDa = 2 cm ...
2) plot values on a semi-logarithmic scale (y-axis: logarithmic=kDa; x-axis: linear= migration distance)
3) determine the migration distance of the protein bands
4) now you can determine the size of the proteins by reference of the graph of point 2)

[rayman]

Thank you.

But the x-axis has to be the logarithm of the molecular weight and the y-axis has to be the migrations distance.

Edited by rayman, 31 August 2011 - 04:22 AM.

[bob1]

Oh noes... it's not that hard to switch the axes...