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protein aggregation

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#1 nagmcb



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Posted 16 January 2003 - 03:48 AM

i have problem in thrombin cleavage, where by my desired protein concentration after cleavage will be very less,

even though there is lot of protein bound to beads, and also cleaved by thrombin

the protein of interest eluted was very less, this may be due to protein aggregation or precipitation,

the protein precipitation is very less/negligible

can any one tell how to prevent/inhibit protein aggregation.

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