Dears,
It would be grateful to know do the FBS of non-USA origin really need to heat inactivate.Currently,there are many studies stating pre experimental incubation at 37 degree just alone will inactivate the complement and real time inactivation is a waste of time and money.
I am using both HEK -239 AND B16. Mistakenly I used the heat inactivated serum with a new bottle,where the cells shows to more adherent and required more trypisnisation time.
I am using the cell lines for trafficking studies ,but so far nothing different observed.
Thank you
0
1 reply to this topic
#2
-
- Active Members
-
- 3,047 posts
Hmmm, I think it's working
Posted 29 August 2011 - 05:00 PM
If you normally use inactivated serum, then you should continue to inactivate your serum regardless of origin.
Inactivation is not really time consuming or money wasting at all - 45 minutes at 56 degrees is a pretty short incubation and many labs have 56 degree waterbaths on constantly for keeping medium-agar solutions liquid.
Inactivation is not really time consuming or money wasting at all - 45 minutes at 56 degrees is a pretty short incubation and many labs have 56 degree waterbaths on constantly for keeping medium-agar solutions liquid.
