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Serum heat inactivation


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#1 brand

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Posted 29 August 2011 - 12:49 AM

Dears,

It would be grateful to know do the FBS of non-USA origin really need to heat inactivate.Currently,there are many studies stating pre experimental incubation at 37 degree just alone will inactivate the complement and real time inactivation is a waste of time and money.

I am using both HEK -239 AND B16. Mistakenly I used the heat inactivated serum with a new bottle,where the cells shows to more adherent and required more trypisnisation time.

I am using the cell lines for trafficking studies ,but so far nothing different observed.

Thank you

#2 bob1

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Posted 29 August 2011 - 05:00 PM

If you normally use inactivated serum, then you should continue to inactivate your serum regardless of origin.

Inactivation is not really time consuming or money wasting at all - 45 minutes at 56 degrees is a pretty short incubation and many labs have 56 degree waterbaths on constantly for keeping medium-agar solutions liquid.




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