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Is it possible to raise an antibody against a 11aa peptide?


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2 replies to this topic

#1 rosepolaris

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Posted 26 August 2011 - 03:45 PM

This peptide seems without any predicted secondary structure.

Although, there's a 7aa conserved motif.


Is there other method I can use to detect such a short peptide in the cell? I want to know it's sub-cellular localization.
Any advice is welcome!


Thanks!!

#2 K.B.

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Posted 26 August 2011 - 10:27 PM

I've seen people raising antibodies against 5-7 AA peptides by conjugating them with carrier protein.

#3 doxorubicin

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Posted 31 August 2011 - 01:30 PM

I have made many antibodies using short 10-15AA peptides. KLH couple your peptide prior to immunization. Consider adding a Cys residue to your peptide sequence to allow you to use sulfo-link based resins for antibody purification.




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