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is there any other method better than plate counting?

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#1 penelope11

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Posted 26 August 2011 - 12:17 PM

i want to enumerate one certain bacteria in soil samples. i choose the selective medium so the bacteria of interest can standout. but the problem is i don't know how many bacteria in the soil. normally, i need to do a series of dilution, but there is still a chance that none of the dilutions is good. in that case, i have to redo it. it's so time-consuming annoying. is there any other method? i heard there is an equipment, spiral plater, anyone has used it?

#2 hobglobin

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Posted 26 August 2011 - 12:50 PM

Isn't this done with quantitative PCR now?
  
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#3 penelope11

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Posted 26 August 2011 - 01:25 PM

View Posthobglobin, on 26 August 2011 - 12:50 PM, said:

Isn't this done with quantitative PCR now?
but qPCR can also count dead cells.




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