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is there any other method better than plate counting?

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#1 penelope11

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Posted 26 August 2011 - 12:17 PM

i want to enumerate one certain bacteria in soil samples. i choose the selective medium so the bacteria of interest can standout. but the problem is i don't know how many bacteria in the soil. normally, i need to do a series of dilution, but there is still a chance that none of the dilutions is good. in that case, i have to redo it. it's so time-consuming annoying. is there any other method? i heard there is an equipment, spiral plater, anyone has used it?

#2 hobglobin

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Posted 26 August 2011 - 12:50 PM

Isn't this done with quantitative PCR now?
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.

#3 penelope11

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Posted 26 August 2011 - 01:25 PM

Isn't this done with quantitative PCR now?

but qPCR can also count dead cells.





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