i want to enumerate one certain bacteria in soil samples. i choose the selective medium so the bacteria of interest can standout. but the problem is i don't know how many bacteria in the soil. normally, i need to do a series of dilution, but there is still a chance that none of the dilutions is good. in that case, i have to redo it. it's so time-consuming annoying. is there any other method? i heard there is an equipment, spiral plater, anyone has used it?
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is there any other method better than plate counting?
Started by penelope11, Aug 26 2011 12:17 PM
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2 replies to this topic
#2
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Growing old is mandatory, growing up is optional...
Posted 26 August 2011 - 12:50 PM
Isn't this done with quantitative PCR now?
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
#3
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Posted 26 August 2011 - 01:25 PM
hobglobin, on 26 August 2011 - 12:50 PM, said:
Isn't this done with quantitative PCR now?
but qPCR can also count dead cells.
