Posted 25 August 2011 - 07:54 AM
I'm characterizing a new compound from Maybridge that is supposed to inhibit my kinase, but so far it isn't doing anything at up to 1000X the reported Ki in cell-based assays. I plan to do some in vitro kinase assays to see if the thing works, but I also would like to determine if this compound is entering into cells. The Maybridge datasheet indicates "OK" for human intestinal absorption and "+/-" for BBB, so presumably they have done some testing. Does anyone have a clever assay to test if a chemical is entering into cells? Are there ways to predict if a chemical will enter cells? I suppose I could get lucky and the compound might be fluorescent, which would make tracking it easy. Does anyone know a way to predict which chemicals will exhibit fluorescence?