Hey all,
do you know if it harms IPTG dissolving it in EtOH. Does it still can be used for Induction?
A water bottle was labelled wrong :-(
Thanks in advance
Ivan
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3 replies to this topic
#2
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Posted 25 August 2011 - 04:21 AM
Small amounts of ethanol have minimal effect on E. coli cultures. It is common to add 1 ml of antibiotics in ethanol to a liter of prepared medium with no discernible effect.
#3
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Unlimited ligation works!
Posted 25 August 2011 - 05:33 PM
Did that once, dissolve IPTG in ethanol. That worked okay.
May your PCR products be long, your protocols short and your boss on holiday
#4
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Posted 05 September 2011 - 02:05 AM
Thanks for the replies, the induction worked very well! So seems no difference dissolving it in EtOH or Water. Since it was 8mL in 8L the quantity seemed not affecting the Bacteria as well.
