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problem with pcr product


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#1 teerachild

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Posted 04 February 2002 - 11:29 PM

My PCR reaction worked but I got another problem. After PCR, I checked my product but gel electroporesis.
Yes, I got my product but also other three unrequired bands. And I have to run gel again to seperate the required band, then sequencing. I think my annealing temperature was very low, 53 C. And I'm afraid tha tI 'll get nonspecific product. Should I increase the annealing temp. to 58 -59 C  to get rid of these nonspecific bands
Thank you.

#2 Cthulhu

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Posted 05 February 2002 - 06:45 AM

Based on the information in the previous post alone: Yes. Try 60 C, it's a standard annealing temperature.




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