Weird DNA electrophoresis resultdna electrophoresis
Posted 24 August 2011 - 08:04 AM
Posted 24 August 2011 - 05:13 PM
Posted 24 August 2011 - 11:27 PM
The three bands between 1 Kb and 2 Kb can't be all undigested plasmid. They are all brighter than the 4.6 Kb band. I just did this experiment again from plasmid isolation. This time the result is quite nomal.
You may have underdigested it - so some of it is still in plasmid form rather than linear fragments.
Edited by clwop, 24 August 2011 - 11:28 PM.
Posted 25 August 2011 - 09:05 PM