I am planning on performing some maxipreps using Qiagens EndoFree kit and I had a few questions. The current rotor for our high performance centrifuge fits the 50ml round bottom oakridge tubes. I currently have some new 50ml polycarbonate and polyproplyene tubes that I will use for my plasmid isolation. The manufactures insist that the fill volume must be at 80% capacity (33.6ml) in order to insure proper performance.
A couple of steps in the maxiprep protocol have you spin in a total volume of 25ml and 5ml(precipitation step) at 15,000 x g. This is well under the 80% capacity. Have any of you ever encountered this problem and what did you do to fix it?
Edited by BSM, 23 August 2011 - 05:09 PM.