1 reply to this topic

[olio]

Why do my samples have gooey DNA after I add Sample buffer. It is still there after boiling the samples. First the lysate is clear when incubated with lysis buffer and I can make it clear by adding more lysis buffer but adding sample buffer will again make the DNA gooey?

I keep everything on ice and incubate with lysis buffer for 5min on ice and add ice cold Sample buffer and boil for 10min.

[protolder]

Hola, you have add loading buffer to whole cells, and whole DNA shows this aspect and difficults loading. So if you can, sonicate with a fine neddle apparatus for for 2-3 seconds each sample. or load the samples in hot directly from the heater, or digest your DNA before adding loading buffer with any DNAse or restriction enzyme. Buena suerte